autoclavable bioreactors

Introduction
applikonIn the laboratory bioreactor and fermentor range Applikon is worldwide market leader because of its dependable and easy to use systems. The bioreactors and fermentors excel in quality and modularity. An Applikon laboratory system is easy to upgrade if a change in research activities occurs. Applikon offers glass autoclavable bioreactors for cell culture applications and glass autoclavable fermentors for microbial culture applications. The systems are built according to the specific demands of a process using an extensive array of standard components. Because of the modularity and flexibility, the user can always adapt the systems to changed process demands. This results in low initial investment and low running costs. The stirred tank reactor (STR) is the most widely used bioreactor type. Glass autoclavable bioreactors and fermentors are available in 1 - 2 - 3 - 5 - 7 - 15 and 20 liter total volume.

Specifications
Autoclavable bioreactor specifications:

 

Total volume (liter)

Working volume (liter)

Minimum working volume (liter)

Aspect ratio total volume

Aspect ratio working volume

 1 liter

1

0.9

0.3

1.9

1.5

 2 liter single wall

 2 liter jacketed

2

2

1.7

1.7

0.5

0.5

2.2

1.9

1.9

 3 liter single wall

 3 liter jacketed

3

3

2.7

2.7

0.5

0.5

1.7

1.5

1.5

 5 liter single wall

 5 liter jacketed

5

5

3.4

3.4

2.0

2.0

1.6

1.1

1.1

 7 liter single wall

 7 liter jacketed

7

7

5.4

5.4

2.0

2.0

2.3

1.8

1.8

 15 liter single wall

 15 liter jacketed

15

15

12

12

2.5

2.5

1.9

1.5

1.5

 20 liter single wall

20

16

2.5

2.5

2.0

 Drive system

Direct drive, lipsealed or magnetically coupled

 Maximum stirrerspeed (rpm)

Standard range is 50 - 1250.

1, 2 and 3 liter systems can be supplied with 2000 rpm motor

 Impellers

Rushton and marine with outside diameters 45mm, 60 mm 75 mm or 85 mm

 Gas sparger

Porous sparger or L-type sparger

 Gas overlay

Yes

 Exhaust gas

Water cooled exhaust gas condenser

 Sampling

Fixed height or height adjustable sample pipe with optional sampling system

Sample pipe internal diameters choices are: 1.7 mm, 4 mm, 6 mm or 10 mm

 Draining

Drain pipe

 Additions

Triple or single inlet ports and optional micro liquid injectors

 pH

Measurement: 12 mm classic pH sensor

Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump

 DO2

Measurement: 12 mm classic polarographic DO2 sensor

Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation or nutrient addition pump

 Temperature

Measurement: Pt-100 sensor in thermowell in topplate

Control: cooling and/or heating jacket via bioreactor wall or via internal heat exchanger

 Foam

Measurement: Height adjustable conductivity based foam sensor

Control: Anti foam addition pump

 Level

Measurement: Height adjustable conductivity based level sensor

Control: pump for liquid addition or removal

 Optional inlets

Septum, chemostat tube, liquid entry system

Applications
Microbial and Cell culture configuration
Batch, Fed-Batch, Perfusion and Continuous cultivation

Features of the glass autoclavable bioreactors:

  • Wide range of volumes with interchangeable modules
  • Wide range of modules to tailor the systems to the research demands
  • No welded parts in the bioreactor topplate
  • Simple set-up and easy to handle
  • Optional high torque magnetically coupled agitator
  • All metal parts are constructed of stainless steel 316L to guarantee resistance to pitting and crevice corrosion in chloride environments
  • Mirror polished finish of non product contact stainless steel surface
  • Electropolished finish of product contact stainless steel parts ease cleanability
  • Glass dished bottom vessels are made of borosilicate glass to guarantee:
    • the best resistance to thermal shock
    • excellent corrosion resistance
    • smooth, non porous surface for easy cleaning
    • optimal transparency for visual inspection of the culture
  • Glass bioreactor vessels can be used up to 0.5 barg (7.25 psig) of overpressure.

Our Location

Contact

RTD Technology Ltd T/A Asistec
Unit 15A, Solus Tower Estate
Corke Abbey, Bray
Co. Wicklow, A98 X9X7
Tel:  +353  (0)1 281 4650
Tel:  +353  (0)1 281 4064
Fax: +353  (0)1 204 3064


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